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. 2014 Feb 18;9(2):e89435. doi: 10.1371/journal.pone.0089435

Figure 7. TUNEL and DAPI staining of cross sections in pupal wings.

Figure 7

TUNEL assays (green: A, C, E, G) and DAPI staining (cyan: B, D, F, H) of cross sections of female and male pupal wings. (A) Cross section of female pupal wing on Day 4 after the beginning of pupal-adult development. (C) Cross section of female pupal wing on Day 6 after injection of 5.4 µg 20E. (E) Cross section of male pupal wing on Day 7 after beginning of pupal-adult development. (G) Cross section of male pupal wing on Day 8 after injection of 5.4 µg 20E. Note that TUNEL signals (light green) indicated by white arrows were visible in areas of the female wing epithelium. Scale bar  = 50 µm.