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. 2013 Dec 17;136(2):660–670. doi: 10.1021/ja406831n

Figure 7.

Figure 7

Lack of hIAPP inhibition by rIAPP. (a) ESI-IMS-MS driftscope plot of oligomers present at t = 2 min in a mixed sample of hIAPP and rIAPP at a 1:1 molar ratio (50 μM final peptide concentration, 20 mM ammonium acetate buffer, pH 6.8, 25 °C). Extracted driftscope peak shows masses corresponding to a mixed dimer of one hIAPP monomer plus one rIAPP monomer (inset). (b) ThT fluorescence intensity during agitation of hIAPP:rIAPP 1:1 (50 μM final peptide concentration, 20 mM ammonium acetate buffer, pH 6.8, 37 °C, 600 rpm). Data for three replicates are shown. (c) Negative stain TEM image of 1:1 hIAPP: rIAPP fibrils after 5 days. Scale bar = 100 nm. (d) ESI mass spectra of depolymerized fibrils showing the presence of both hIAPP and rIAPP monomer constituents in the pellet (i) and supernatant (ii) following ultracentrifugation.