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SDS-PAGE analysis of AtMan5-1. a AtMAN5-1e: lane 2, crude extract before purification; lane 3 and lane 4, 0.7 and 2.2 μg purified recombinant protein, respectively. b AtMan5-1p: lane 2 crude extract before purification; lane 3 9.6 μg purified recombinant protein before deglycosylation with PNGase F; lane 4 9.6 μg purified recombinant protein after deglycosylation with PNGase F
