Fig. 5.
Total contents of S-nitrosothiols (SNOs) in roots (a), stems (b) and leaves (c) of potato cv. ‘Bintje’ treated with 250 μM AlCl3 or without AlCl3 (acidic control) at 48 h. Nitrosothiol content was determined by chemiluminescence using a Sievers® Nitric Oxide Analyzer NOA 280i. Detection of SNOs in potato leaves by immunofluorescence histochemistry using Alexa Fluor 405 Hg-Link reagent phenylmercury. Blue fluorescence attributable to SNOs in roots (d, e) and leaves (f–j) of control and Al-treated potato. Bars indicate 250 μm (d, e), 200 μm (h, i), 100 μm (f, g, j). Asterisks indicate values that differ significantly from non-treated control potato plants at P < 0.05