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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2013 Nov 26;46:138–147. doi: 10.1016/j.biocel.2013.11.015

Fig. 5.

Fig. 5

Cytokines stimulate motility of SUM149 IBC cells. (A) Cells were seeded in media containing MCP-1/CCL2, TNF-α, IL-8 or IL 10 and cell motility was measured by a wound healing assay as described in Section 2. Area of wound closure was imaged at the indicated times and measured using ImageJ. Scale bar= 100 µm. (B) Graph illustrates the percentage of wound closure. TNF-α, IL-8 and IL-10 significantly enhance motility of SUM149 cells. Results are expressed as mean ± S.D of three independent experiments; *represents p≤ 0.00 as determined by Fisher’s least significant difference (LSD).