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. 2014 Feb 19;5:46. doi: 10.3389/fpls.2014.00046

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Copyright © 2014 Maeda, Song, Sage and DellaPenna.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Whole plant and vascular callose phenotypes of Col, vte2, gsl4, gsl4 vte2, gsl11, and gsl11 vte2. All genotypes were grown under permissive conditions for 4 weeks and then transferred to LT conditions for the specified periods previously shown to maximize each phenotype (Maeda et al., 2006). (A) Whole plant phenotype of the indicated genotypes before (top) and after (bottom) 28 days of LT treatment. Bar = 2 cm. (B) Aniline-blue positive fluorescence in the lower portions of leaves after 3 days of LT treatment. Samples for callose staining were fixed in the middle of the light cycle. Representative images are shown (n = 3). Bar = 1 mm.