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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Mar 29;1833(8):1820–1831. doi: 10.1016/j.bbamcr.2013.03.021

Figure 3. Co-immunoprecipitation of GluR1 and cGKII from the rat cerebellum.

Figure 3

Solubilized extracts of the rat (P14) cerebellum were immunoprecipitated using a mouse anti-flag antibody (2 μg, lane 1), rabbit anti-cGKII polyclonal antiserum (2 μg, lane 2), rabbit anti-HA antiserum (2 μg, lane3), a rabbit anti-GluA1 polyclonal antiserum (2 μg, lane 4) or a mouse anti-GluA1 monoclonal antibody (2mg, lane 4 of cGKII IB). Extracts (Crude) and immunoprecipitates (IP) were analyzed in immunoblots probed with a rabbit anti-GluA1 polyclonal antiserum (1 μg/ml) and rabbit anti-cGKII (1 μg/ml) antiserum. HRP-conjugated anti-rabbit IgG and anti-mouse TrueBlot™ (1:1000) antibodies were used as secondary antibodies to avoid IgG cross-reactivity. The immunoreactive bands were visualized by chemiluminescence.