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. 2014 Feb 19;5:37. doi: 10.3389/fpls.2014.00037

Figure 2.

Figure 2

Localization of rice SAUL-type PUB-ARM proteins at the plasma membrane. (A) Localization of GFP-OsPUB23 fusion proteins at the plasma membrane of Arabidopsis protoplasts. Confocal laser scanning microscopy detected fluorescence of GFP-OsPUB23 proteins depicted in green at the plasma membrane of protoplasts co-expressing GFP-OsPUB23 and AtINT4-RFP. Chlorophyll auto-fluorescence within the chloroplasts is depicted in blue. (B) Localization of AtINT4-RFP fusion proteins at the plasma membrane. Confocal laser scanning microscopy detected fluorescence of AtINT4-RFP proteins depicted in red at the plasma membrane of protoplasts co-expressing GFP-OsPUB23 and AtINT4-RFP. Chlorophyll auto-fluorescence within the chloroplasts is depicted in blue. (C) Merged image of (A,B). Yellow signals derived from the overlap of green and red fluorescences from (A,B). (D) Bright filed image of the protoplast analyzed in (A–C). (E) Localization of GFP-OsPUB21.1 fusion proteins at the plasma membrane. Confocal laser scanning microscopy detected fluorescence of GFP-OsPUB21.1 proteins depicted in green at the plasma membrane of transformed protoplasts. Chlorophyll auto-fluorescence within the chloroplasts is depicted in red. (F) Localization of GFP-OsPUB21.2 fusion proteins at the plasma membrane. Confocal laser scanning microscopy detected fluorescence of GFP-OsPUB21.1 proteins depicted in green in the cytosol of transformed Arabidopsis protoplasts. Chlorophyll auto-fluorescence within the chloroplasts is depicted in red.