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. Author manuscript; available in PMC: 2014 Mar 3.
Published in final edited form as: Circulation. 2012 Oct 3;126(15):1882–1895. doi: 10.1161/CIRCULATIONAHA.112.113290

Figure 4. Quantification of infiltrating circulating cells in Cxcl12Gagtm/Gagtm animals.

Figure 4

Percentage of CD45.1+, CD45.1+/CD34+ and CD45.1+/CXCR4+ in blood (A) and ischemic muscle (B), 6 days after the onset of ischemia. Representative FACS dot plots obtained from WT and MUT digested muscle at 6 days after ischemia. Cells were gated on CXCR4 expressing CD45.1-positive cells. n = 7 per group. A–B) 1 possible comparisons for Bonferroni correction, a value of p<0.05 was considered significant. *p<0.05 versus WT. (C) Quantitative evaluation and representative photomicrographs of Mac3-positive cells (macrophages in brown, arrows show Mac-3 labelled macrophages) in Cxcl12Gagtm/Gagtm (MUT) and wild-type littermate (WT) animals. Results are shown in ischemic (Isc) and non-ischemic (N.Isc) legs. Values are mean ± SEM. n = 15 per group, representative of 3 independent experiments. 6 possible comparisons for Bonferroni correction, a value of p<0.008 was considered significant, **p<0.0016 versus Isc WT.