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. Author manuscript; available in PMC: 2014 Jul 11.
Published in final edited form as: Cell Rep. 2013 Jul 3;4(1):87–98. doi: 10.1016/j.celrep.2013.05.045

Figure 3. Effect of TFIIIA Depletion on Ribosome Biogenesis.

Figure 3

(A) Pre-rRNA processing pathway in mammals.

(B) EB-stained agarose gel (left) and autoradiogram of a northern blot (right) of 3H-uridine-labeled RNA (1 hr pulse and 4 hr chase) from U2 OS cells 72 hr after the transfection of si-NS or si-TF; 1 μg of total cellular RNA per lane.

(C) EB stained agarose gel (left) and northern blot (right) of total RNA extracted from U2 OS cells 72 hr after transfection with siRNAs specific for si-NS (NS), si-TF (TF), RPL5 (L5), or RPL11 (L11). Northern blot hybridized with a probe directed against ITS2 (internal transcribed spacer 2) of precursor rRNA: 1 μg of total cellular RNA per lane.

(D) Polysome profiles of extracts from U2 OS cells transfected with si-NS or si-TF.