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. Author manuscript; available in PMC: 2014 Jul 11.
Published in final edited form as: Cell Rep. 2013 Jul 3;4(1):87–98. doi: 10.1016/j.celrep.2013.05.045

Figure 4. Effect of TFIIIA Depletion on the Stabilization of p53.

Figure 4

(A) U2 OS, HCT 116, and A549 cells were transfected for 72 hr with si-NS or si-TF, total RNA was extracted, and TFIIIA mRNA levels were evaluated by quantitative real-time PCR analysis. Bar graphs show the mean ±SEM of three independent experiments.

(B) Western blot analysis showing expression levels of p53, p21, and Hdm2 and the loading control β-actin in U2 OS, A549, and HCT 116 cells 72 hr after transfection with si-NS, si-TF, or an siRNA specific for POLR1A (Pol I).

(C) Cell-cycle phase distribution in U2 OS cells transfected with the indicated siRNAs.

(D) Western blot analyses showing expression levels of p53, p21, and Hdm2 and the loading control β-actin in U2 OS cells transfected for 72 hr with individual or combined siRNAs to NS, TFIIIA, RPL5, RPL11, or Pol I.

See also Figures S2 and S3.

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