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. 2014 Feb 17;13:25. doi: 10.1186/1475-2859-13-25

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Copyright © 2014 Castro-Acosta et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterization of purified VP6_NT. A) 12% SDS-PAGE gel stained with Coomassie Blue (under reducing conditions): Lane 1, Molecular weight marker Benchmark (Life Technologies Corp., Carlsbad, CA, USA), the more intense band corresponds to 50 KDa. Lane 2, VP6_NT. B) Size exclusion chromatography, Peak 1 corresponds to VP6_NT, peak 2 to VP6_U and peak 3 to salts. K_D refers to the relative elution volume calculated with Equation 1. C) Transmission electronic micrograph at 85,000X. D) Intrinsic fluorescence spectrum of 40 µg/mL of VP6_NT or VP6_U. Excitation at 280 nm.