Figure 2.

Densitometric analysis of reducing 12% SDS-PAGE gels loaded with 2.5 µg of VP6_NT, VP6_U and BSA previously exposed to different oxidative treatments and stained with Coomassie blue. A) Exposure of protein samples to different concentrations of H₂O₂ for 6 h. B) Exposure of protein samples to metal catalyzed oxidation (MCO) with 150 µM of FeCl₂ at various H₂O₂ concentrations for 1 h. C) Exposure of protein samples to MCO with 150 µM of FeCl₂ at various H₂O₂ concentrations for 6 h. Measurements were performed to identically treated samples from triplicate experiments. Error bars represent standard deviations among experiments.