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. 2014 Feb 17;13:25. doi: 10.1186/1475-2859-13-25

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Copyright © 2014 Castro-Acosta et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Emission fluorescence scans at λ_ex295 nm of VP6_NTand VP6_U(0.4 mg/mL) after MCO with 150 µM of FeCl₂at various H₂O₂concentrations for 1 h. A) Normalized emission spectra of VP6_NT. B) Normalized emission spectra of VP6_U. C) The center of spectral mass (CSM) of oxidized VP6_NT and VP6_U was calculated using Equation (3) and plotted against the H₂O₂ concentration used. On the right, the CSM of denatured VP6 (treated for 2 hours with 6 M of guanidine chloride) is shown for comparison. Experiments were performed in triplicate, except for the CSM of denatured VP6, which was analyzed in duplicate. Error bars represent the standard deviation or difference among them.