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. Author manuscript; available in PMC: 2015 Apr 3.
Published in final edited form as: Prog Neuropsychopharmacol Biol Psychiatry. 2013 Dec 12;50:53–65. doi: 10.1016/j.pnpbp.2013.12.003

Figure 7.

Figure 7

Interaction of MOR and WLS in the locus coeruleus (LC) of saline and heroin self-administering rats. (A) MOR was immunoprecipitated (IP) from the LC of saline (control) and heroin (drug) self-administering rats using a rabbit anti-MOR antibody. Equal amounts of protein from LC tissue were added to each immunoprecipitation reaction. Interaction with WLS was determined by immunoblotting (IB) with a chicken anti-WLS antibody. Lysate (L) lanes contain 1% of the total protein from the LC compared to the mock (M, rabbit IgG) and immunoprecipitation (IP) lanes. Arrow indicates position of WLS. IgG heavy chain migrates as a dimer at ∼100 kDa. (B) Bands were analyzed by densitometry and quantified using Image J software. Data was analyzed using paired Student’s t-test (expressed as a mean ± SEM; n=3, (*P < 0.05).