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. 2014 Feb 11;11:25. doi: 10.1186/1743-422X-11-25

Table 3.

Primers for specific and long-distance PCR

PCR	Gene	HAdV species	1^st-round primer (5′-3′)		2^nd-round primer (5′-3′)		3^rd-round primer (5′-3′)		Annealing temp. (C°)	Fragment size
PCR	Gene	HAdV species	Sense	Anti-sense	Sense	Anti-sense	Sense	Anti-sense	Annealing temp. (C°)	Fragment size
Generic PCR	Hexon	HAdV-D	AAGGCCGTCA	GTGCGGCTGG	ACAACTCGGGCTTCACCGGC	GGCTGGTGCA	None	None	63	322 bp
Generic PCR	Hexon	HAdV-D	CCCTGCCCTT	TGCACTCTGA	ACAACTCGGGCTTCACCGGC	CTCTGACCACG	None	None	63	322 bp
Long distance PCR	PVII-Hexon	HAdV-D	CGCCCAGCAA	GTGCGGCTGG	GGCAGGACTCGCAGACGAGC	GGTGGGCTCA	GCGCGGAAAC	GGGATGCGCC	60	4.8 kb
			TAACACCGGC	TGCACTCTGA		TCCATGGGGT	GTGTACTGGGT	ACATGACCCT