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. 2014 Jan 15;21(1):4. doi: 10.1186/1423-0127-21-4

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Copyright © 2014 Chae et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The effect of HPB242 on apoptosis of HN22 and HSC4 cells. HN22 (A) and HSC4 (B) cells were incubated with HPB242 (0, 5, 10 and 20 μg/ml) for 48 h. Fifty micrograms of cellular extract per lane was separated on an SDS-PAGE gel as described in Methods. Immunoblot detection of Bid, Cleaved-caspase3, and PARP, upregulation of Bax, downregulation of Bcl-_xl in whole cell lysates from various concentration HPB242-treated HN22 and HSC4 cells for 48 hours. Equal loading of protein was confirmed by incubating the same membrane with anti-β-Actin antibody.