. 2014 Jan 18;15(1):38. doi: 10.1186/1471-2164-15-38

Table 5.

Distribution of Hif KR494 unique genes in clinical Hif isolates

Locus tag ^a (HifGL)	Description ^b	*H. influenzae* serotype f clinical isolates
Locus tag ^a (HifGL)	Description ^b	KR494	G19	G20	K238	L11	L16	L21	L22	L24	L25	L29	L45	L50	L59	M1	M10	M14	M29	M54	S208	S229	No. of isolate ^c	Hib MinnA	NTHi 3655
000176	cw-hydrolase	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+	19	+^d	+^d
000310	GST	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	20	-	-
000674^a	RgD_F1: sap2	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	19	-	-
000799^a	KNTase	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	20	-	-
000834^a	RgD_F 2: glpR	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+	19	-	-
000837	TAA	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	20	-	-
000989^a	RgD_F 3: aef3	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	20	-	-
001007^a	RgD_F 3:PI	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	-	+	+	18	-	-
001350^a	RgD_F 4: rnf	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	-	+	+	+	+	+	18	-	-
001363^a	RgD_F 4: PI	+	+	+	+	+	+	+	+	+	+	+	+	+	+^e	-	+	+	+	+	+	+	19	-	-
001431	TAA	+	-	-	+	+	-	+	+	-	+	+	-	+	-	+	-	-	+	+	+	+	12	-	-
001444	Fe³⁺ transporter	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	20	-	-
001463	UHP	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	20	-	-
001635	RgD_F 6: RHinfI	+	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	19	-	-
001636	RgD_F 6: MHinfI	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+	19	-	-
001664	HutZ	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	20	-	-
001672	Lpp	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	20	-	-
No. of CDS present		17	16	15	17	17	16	17	17	16	17	17	16	17	13	14	14	16	17	16	17	17		1	1

^aLocus tags were based on the Hif KR494 genome [15]. For PCRs targeting the contiguous loci, only the first locus is shown. Full information of the target genes, amplicon size and PCR conditions is described in Additional file 1.

^bAbbreviations: cw-hydrolase, cell wall-associated hydrolase; glpR, duplication of gene cluster involved in sugar and amino acid transport and metabolism; GST, glutathione S-transferase domain-containing protein; KNTase, kanamycin nucleotidyltranserase; Lpp,unknown lipoprotein; MHinfI,type f-specific adenine-specific methyltransferase HinfI; PI, prophage island; TAA, trimeric autotransporter; UHP, unknown function hypothetical protein; RHinfI,Type II restriction enzyme HinfI.

^cTotal number of Hif clinical isolates (excluding Hif KR494) with unique genes found in Hif KR494.

^dCDS was not found despite the presence of primer priming site in the NTHi 3655 genome (AAZF01000000) and Hib 10810 (NC_016809); PCR product had a smaller size.

^ePositive, but with a larger PCR product.

⁺PCR positive (CDS present); ^-PCR negative (CDS absent).