Table 6.

Distribution of genes absent in Hif KR494 and other Hif clinical isolates

Locus a	Description/gene b	Serotype f clinical isolates
		KR494	G19	G20	K238	L11	L16	L21	L22	L24	L25	L29	L45	L50	L59	M1	M10	M14	M29	M54	S208	S229	No. of isolate c	Hib MinnA	NTHi 3655
Histidine biosynthesis
HI0468	hisG	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	2	+	+
HI0469	hisD	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	3	+	+
HI0470	hisC	-	+	+	-	+	-	+	-	+	-	+	-	+	+	+	+	+	-	-	+	-	12	+	+
HI0472	hisH	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	2	+	+
Anaerobic fermentation of L-ascorbate
HI1024	ulaD	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	1	-d	-d
Folate biosynthesis
HI1190	PTS	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	1	+	+
Zinc and secondary molybdenum transporter
HIB_07090	zitB	-	-	+	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	5	+	+
HI1470	molC	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	2	+	-d
HI1472	molA	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	0	+	-d
hmg locus involved in LOS glycoform
HI0871	siaA	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	0	+	+
HI0872	wbaP	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-		-	-	-	0	+	+
No. of CDSs present		0	2	5	0	1	0	1	0	1	0	1	0	1	3	5	3	1	0	0	3	1		10	8

^aLocus tags were based upon the genome of Rd Kw20 except HIB_07090 is based on Hib 10810. For PCR targeting the contiguous loci, only the first locus is shown. Full information of the target genes, amplicon size and PCR conditions is described in Additional file 1.

^bAbbreviations: PTS, 6-pyruvoyl tetrahydrobiopterin synthase in folate biosynthesis.

^cTotal number of Hif clinical isolates (excluding KR494) with genes that were absent in Hif KR494.

^dCDSs that were present in Rd Kw20 but not in NTHi 3655 or Hib MinnA.

⁺PCR positive (CDS present); ^-PCR negative (CDS absent).