Figure 2.

Effects of various treatments in vitro on PER immunofluorescence in PG cells (A–F), FB cells (G–L), and SG cells (M–R) from arrhythmic LL animals at day 12 after feeding. Fluorescence in shown as green/yellow. Arrows in (A,G,M) show nuclei. Controls were incubated in light. In PG cells note the substantial increase in nuclear immunofluorescence intensity following all treatments compared to controls. Note that transfer to dark and treatments with bombyxin or PTTH had no effect in FB and SG cells. Note that treatment with brain extract, which contains both ILP and PTTH, and combination treatment with both bombyxin (an ILP) and PTTH increased substantially both nuclear and cytoplasmic fluorescence intensity in all three cell types when compared with other treatments. Scale bars = 10 μm.