Expression of ZnT2 in choroid plexus tissue and effect of Pb exposure. A(a–c). Choroid plexus tissues were dissected from the lateral ventricles. A(d–f). For in vivo Pb exposure, animals received a single ip injection of 50 mg PbAc for 24 h. A(a) and A(d) show the ZnT2 green fluorescent signal; A(b) and A(c) show the DIC mages; A(c) and A(f) are the merged images of ZnT2 fluorescent signal and DIC. (B) qPCR quantitation of ZnT2 mRNA expression in the choroid plexus. Data represent mean ± SD, n=6, *P < 0.05, as compared with the control. (C) Western blot analysis of ZnT2 in the choroid plexus. β-actin was used as the house-keeping protein. (D) Quantification of western blot was expressed as the OD ratio of ZnT2/β-actin. Data represent mean ± SD, n=3, *P < 0.05 as compared with the control. (A color version of this figure is available in the online journal.)