Presence of ZnT2 in choroidal Z310 cells and effect of Pb exposure. A(a–c)) Control cells; A(d–f) Pb-treated Z310 cells. A(a) and A(d) show the ZnT2 green fluorescent signal; A(b) and A(c) show the DIC images; A(c) and A(f) are the merged images of ZnT2 fluorescent signal and DIC. (B) qPCR quantitation of ZnT2 mRNA expression. Data represent mean ± SD, n=6, **P < 0.01 as compared with the control. (C) Western blot analysis of ZnT2 in Z310 cells. β-actin was used as the internal control. (D) Western blot quantification. Data represent mean±SD, n=3, *P<0.01, as compared with the control. (A color version of this figure is available in the online journal.)