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Iodoacetamide labeling for azoline localization. In an ordered fashion, the cyclodehydratase installs azoline heterocycles onto the precursor peptide. Cysteines that have not undergone cyclodehydration were labeled with iodoacetamide. Acid-catalyzed hydrolysis reverts thiazolines back to cysteines. Localizing the unmodified and acetamide-labeled cysteines allowed the order of processing to be determined.
