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Expression of LovD from E. coli BL21(DE3)/pAW31. The soluble and insoluble portions of LovD expressed from E. coli at different temperatures were examined using SDS-PAGE. M: Invitrogen Benchmark Ladder; S: soluble fraction; I: insoluble fraction. The gel shows that LovD is prone to misfolding and aggregation, and even at lower expression temperatures, more than 50% of LovD remains insoluble.
