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. Author manuscript; available in PMC: 2014 Feb 19.
Published in final edited form as: Stem Cells Dev. 2011 Apr 6;20(11):1817–1827. doi: 10.1089/scd.2011.0042

FIG. 5.

FIG. 5

Maintenance of the Noto-GFP+ cell population. NotoGfp/+ cells were differentiated using the 2-step differentiation protocol followed by a third step in the presence of 100 ng/mL Fgf2, 100 ng/mL Shh, or 25 ng/mL Wnt or combinations of them. Additionally, culture was continued in step 2 medium. (A) Schematic view of culture procedure. (B) FACS analysis of Noto-GFP expression was performed at day 5 to 8. (C) In the same setup the absolute cell number per well was counted. (D) Based on percentage GFP+ and absolute cell number per well, the absolute number of Noto-GFP+ cells per well was determined. The data represent the mean ± SD of 3 independent experiments.