Fig. 2.

Effects of AEMP on histamine release and intracellular calcium.

(a) HMC-1 cells (2×10⁵ cells/ml) were preincubated with various concentrations of AEMP at 37° for 30 min prior to incubation with PMA (20 nM) and calcium ionophore A23187 (1 μM). Histamine content was measured by spectrofluorometer. (b) Intracellular calcium was detected by fluorescence microplate leader. (c) Cells were treated with various concentrations of AEMP (1-1000 μg/ml) for 24 h. The cell viability was determined using the MTT assay. Cell viability was determined by the relative absorbance, compared with control. Each data represents the mean±SEM of three independent experiments. *Significant difference at P<0.05.