Fig. 4.

Effects of AEMP on the activation of NF-κB.

HMC-1 cells were pre-treated with AEMP for 30 min prior to PMA (20 nM) and A23187 (1 μM) stimulation for 2 h. (a) Nuclear translocation of NF-κB was assayed by Western blot (N-NF-κB, nuclear NF-κB). (b) Cells were transiently transfected with the NF-κB-luciferase reporter construct or empty vector. Then, the cells were incubated with PMA and A23187 with or without AEMP. NF-κB-dependent transcriptional activity was determined by luciferase activity assay. Each bar represents the mean±SEM of three independent experiments. *Significant difference from PMACI value at P<0.05.