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Gating strategy for the determination of PMNL. Representative FACS diagrams are shown. In (a), blood samples, obtained from cis-NF-κBEGFP after feeding Lieber-DeCarli diet for 4 weeks, were analyzed and the region (R2) was set according to the forward/side scatter characteristics (FSC/SSC) of PMNL. Dot plots depict EGFP expression versus isotype control CD11b staining from (b) pair- and (c) ethanol-fed mice.
