Figure 8.

NF-κB target gene expression in isolated Kupffer cells in response to TNF-α after ethanol feeding F4/80⁺ macrophages were isolated from livers of ethanol-, pair-fed mice, respectively, and stimulated with TNF-α (500 ng/mL) for 2 h and 24 h. Supernatants were measured for mRNA levels of IL-6 (a), TNF-α (b), NOS2 (c), CXCL-1 (d), and MMP-9 (e) by RT-PCR. Data (mean ± SEM) are expressed as fold change compared with corresponding medium controls (unstimulated) and are representative of five to eight separate experiments. *P < 0.05 versus pair-fed control.