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. 2014 Jan 20;24(2):181–186. doi: 10.1016/j.cub.2013.11.044

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© 2014 The Authors

This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/3.0/).

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Morphology of 2K1N Promastigote-like Trypanosomes

(A and B) Differential staining of DNA with DAPI and PI. DAPI preferentially binds to AT-rich DNA compared to PI, allowing the AT-rich kinetoplast DNA to be distinguished from nuclear DNA. Two different 2K1N PL cells are shown.

(C) Localization of protein P166 from tripartite attachment complex in 2K1N PL cell of T. b. brucei J10 carrying fusion construct YFP::P166.

(D) Visualization of flagellum in 2K1N PL cell of T. b. brucei J10 carrying fusion construct YFP::PFR1. The trypanosome has a single flagellum associated with the anterior kinetoplast. Scale bars, 10 μm. See also Figure S1.