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. 2014 Jan 20;24(2):181–186. doi: 10.1016/j.cub.2013.11.044

Figure 4.

Figure 4

Nuclear and Kinetoplast DNA Contents of Salivary Gland-Derived Trypanosomes

Trypanosomes (1738 GFP) from flies dissected 17–20 days after infection were fixed and stained with DAPI and PI before imaging under uniform conditions. Total pixel intensities were measured for both DAPI and PI-stained nuclei and kinetoplasts using ImageJ (http://rsb.info.nih.gov/ij) and normalized relative to G1-arrested metacyclics (META, nuclear DNA content = 1.0; kinetoplast DNA content = 1.0). Other cells were categorized by morphology into promastigote-like cells (PL; see Figure 2C), procyclics (PRO; long trypomastigote with round or oval nucleus), epimastigotes (EPI; nucleus posterior to kinetoplast), cells in meiosis I (MEI; epimastigote with posterior nucleus, two anterior kinetoplasts and associated flagella). N denotes number of organelles measured in each cell category.

(A) Nuclear DNA contents. Each histogram shows the frequency distribution of total normalized pixel intensities per nucleus; values on the x axis are the median for each bin (bin size = 0.25). The superimposed dotted line is a smooth curve.

(B) Kinetoplast DNA contents. Each histogram shows the frequency distribution of total normalized pixel intensities per kinetoplast; values on the x axis are the median for each bin (bin size = 0.25).