Table 1.
Nucleotide sequences of primers and probes used for real-time PCR
| Gene | Nucleotide sequence of primers and probes (5′–3′) | Accession number |
|---|---|---|
| SREBF1 | ||
| Forward | CGGACGGCTCACAATGC | AB686492 |
| Reverse | GCAAGACGGCGGATTTATTC | |
| Probe | TCAACGACAAGATCATCGAG | |
| PPARγ1 | ||
| Forward | CTCGGACACCGGAGCTGG | AJ006756 |
| Reverse | CAACCATGGTCACCTCGCTAA | |
| Probe | CGCCAGGCCACCACCGCAGATT | |
| PPARγ2 | ||
| Forward | GGTGAAACTCTGGGAGATTCTCTTA | AF059245 |
| Reverse | CAACCATGGTCACCTCTTGTGA | |
| Probe | CGATGCCTTCGACACGCTGTCTGCAA | |
| C/EBPα | ||
| Forward | AGGAGGACGAGTCGAAGCA | XM_003127015 |
| Reverse | GGCGGAGGGTGTGAATGC | |
| Probe | CTTTCCCTACCAGCCACCGCCGC | |
| EBF1 | ||
| Forward | ATGTTTGTCCATAATAACTCCAAGC | XM_003359834 |
| Reverse | CTTTGATACAGGGAGTAGCATGTT | |
| Probe | ACCCCTCGGAAGGTACGCCCTCTTATC | |
| FASN | ||
| Forward | GCTGGCCTACACGCAGAG | NM_001099930 |
| Reverse | GGCCCTGGAGCGGTATCA | |
| Probe | CGCCTCCAGCACCTTGCCTTGC | |
| aP2 | ||
| Forward | CAGGAATTTGATGAAGTCACTGC | NM_001002817 |
| Reverse | GTGGTTGTCTTTCCATCCCAC | |
| Probe | TGACAGGAAAGTCAAGAGCACCATAACCTT | |
| ADIPOQ | ||
| Forward | CACCACTGGCAAATTCCACTG | NM_214370 |
| Reverse | CCTTCACATCCTTCAAGTAGACC | |
| Probe | CCTGGGCTGTACTACTTCTCCTTCCACG | |
| CD36 | ||
| Forward | CCTACTGGCTGAGTTATTGTGAC | NM_001044622 |
| Reverse | CACAGCATAGATTGACCTGCAA | |
| Probe | TGGTACAGATGCAGCCTCATTTCCACCT | |
| SCD1 | ||
| Forward | ACGGATATCGCCCTTATGACAAG | NM_213781 |
| Reverse | CGCTGGCAGAATAGTCATAGGG | |
| Probe | TGGAAGCCCTCACCCACAGCTCCC | |