Figure 4.

Interaction of WT ACE and RTD mutants with CNX in human embryonic kidney (HEK) and CHO cells. (A) Confocal analysis in HEK cells: HEK cells, transiently expressing WT and the six different mutants, were fixed 6 h 30 min and 48 h after transfection, permeabilized and stained with the HKCE Ab (green) and a CNX Ab (red). Only merge images are shown. Scale bar 10 µm. (B) Co-immunoprecipitations (IP) of WT and RTD mutants with CNX in CHO cells. IP of cell lysates was performed by incubation with anti-ACE Ab HKCE (IP-ACE) or anti-CNX Ab (IP-CNX). Immune complexes were precipitated with Protein G-Sepharose and analysed by immuno-blot (IB) with anti-ACE Ab Y1 (IB ACE) or anti-CNX Ab (IB-CNX).