Table 1.
Acanthamoeba conditioned medium (ACM) exhibited selective antibacterial properties
| Bacteria + Acanthamoeba conditioned medium (ACM) | CFU remaining a (Bacteria + ACM) | Bacterial kill (%) |
|---|---|---|
| Methicillin-resistant Staphylococcus aureus (MRSA, 10 μL) + ACM (190 μL) |
0 |
100 |
|
Acinetobacter (10 μL) + ACM (190 μL) |
1 × 1010 |
0 |
|
Pseudomonas aeruginosa (10 μL) + ACM (190 μL) |
6 × 109 |
0 |
| Vancomycin resistant Enterococcus faecalis (VRE, 10 μL) + ACM (190 μL) |
3.75 × 105 |
8.12 ± 9.8 |
| MRSA (10 μL) + heated ACM (190 μL) |
0 |
100 |
| MRSA (10 μL) + heated ACM (180 μL) + PYG (10 μL) |
6.65 × 104 |
93.3 ± 9.9 |
| MRSA (10 μL) + heated ACM (140 μL) + PYG (50 μL) |
1.55 × 107 |
0 |
| MRSA (10 μL) + heated ACM eluate of 10 kDa spin exclusion column (190 μL) |
0 |
100 |
| MRSA (10 μL) + heated ACM eluatee of 5 kDa spin exclusion column (190 μL) |
1.34 × 1010 |
0 |
| MRSA (10 μL) + heated-pronase-treated ACM (190 μL) | 0 | 100 |
The data are presented as the mean ± SE of three independent experiments. aAn inoculum of ~106 bacterial c.f.u. was used.