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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Biomaterials. 2014 Jan 4;35(9):3071–3079. doi: 10.1016/j.biomaterials.2013.12.026

Figure 1. Confirmation of siRNA delivery and target mRNA knockdown.

Figure 1

A) Fluorescent microscope images of AoSMC transfected with Control siRNA, siGLO Red or Cholesterol (Chol) siRNA complexed without transfection reagent or with RNAiMax or PEI (N/P ratio of 2.5, 5 or 10) (Mag=10x). B) Thrombospondin-2 (TSP-2) mRNA expression in AoSMC after transfecting with Control siRNA (0.5μg), TSP-2 siRNA (0.25μg or 0.5μg), Chol siRNA (0.25μg) or Chol TSP-2 siRNA (0.25μg or 0.5μg) using PEI.