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. 2014 Feb;64(1):50–54.

Figure 2.

Figure 2.

Guidelines for intradermal immunization of minipigs. (A) Frequent interaction between personnel and minipigs is recommended during the 14-d acclimation period before study inititation. (B) Minipigs can be restrained by holding them. (C) Intradermal immunization requires shaving of the abdomen. (D) Intradermal vaccination by using a 25-gauge, beveled needle requires isoflurane or sevoflurane anesthesia of pigs, to maximize accuracy and minimize stress. (E) Intradermal bleb after intradermal vaccine delivery. (F) Intradermal vaccination by using DSJI device. (G) Blood sampling of minipig in dorsal recumbancy. (H) Incisions in the armpits of a minipig after blood sampling. (I) Resection of the spleen after blood sampling.