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Nuclear translocation of Sox1 during pre-inducing process. (A) Cell morphorlogy at d0, d2, d4, d6, and d8 after cultured in the pre-inducing medium. Real-time PCR (B), western blot analysis (C), and immunostaining (D) of Sox1 expression. Bar=100 μm. All data represent mean±standard deviation, n=3 (*P<0.05 compared with d0).
