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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: Cancer Res. 2013 Dec 5;74(4):1005–1010. doi: 10.1158/0008-5472.CAN-13-1911

Figure 2. ICC is derived from CK19 positive cholangiocytes.

Figure 2

(A) Experimental strategy of tamoxifen induction in CK19CreERTeYFPR26p53f/f mice followed by oral administration of 600mg/ml thioacetamide for 26 weeks. (B) Multifocal tumours developed only in CK19CreERTeYFPp53−/− (homozygous for p53 deletion) (n=5) and not CK19CreERTeYFPR26p53+/− (n=14) or CK19CreERTeYFPR26p53+/+ (n=5) animals, and only following TAA administration. (C) Co-immunofluorescent staining of eYFP with the biliary lineage markers CK19 and Sox9. All eYFP+ cells were seen to be CK19 positive. eYFP positivity did not overlap with the mature hepatocyte marker Cyp2D6. Nuclei are stained with DAPI (Scalebars: 50μm).