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. 2014 Feb 3;2014:978984. doi: 10.1155/2014/978984

Figure 1.

Figure 1

qRT-PCR and western blotting analysis of PinX1 expression in PinX1 overexpressed and knocked-down breast cell lines. (a) Fold changes (2−ΔΔCt values) by qRT-PCR showed increased expression of PinX1 mRNA in the pcDNA3.1-PinX1 transfected breast cancer cell lines MCF-7, MDA-MB-231, and SK-BR-3 when compared with their counterpart untransfected cells and empty vector transfected control cells. Expression levels were normalized for GAPDH. (b) Western blotting indicated upregulation of PinX1 protein in the pcDNA3.1-PinX1 transfected breast cancer cell lines MCF-7, MDA-MB-231, and SK-BR-3 in comparison with untransfected cells and empty vector transfected control cells. (c) Fold changes (2−ΔΔCt values) by qRT-PCR showed decreased expression of PinX1 mRNA in the PinX1 siRNA fragments transfected MCF-10A cells, when compared with the untransfected cells and siRNA NC transfected control cells. (d) Western blotting indicated downregulation of PinX1 protein in the PinX1 siRNA fragments transfected MCF-10A cells in comparison with untransfected cells and siRNA NC transfected control cells.