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HPLC trace of a LPA mixture (10 μM LPA 14:0, 16:0, 18:0, 18:1, 20:4 and 20 μM LPA 17:0). Chromatographic conditions: column: Luna™ C-8, 3 μm, 50 × 2.0 mm; mobile phase: MeOH:phosphate buffer (50 mM, pH 2.5) 16:5; flow rate: 0.32 mL/min; injection volume: 20 μL; sample concentration: 10 μM in MeOH:H2O 9:1; post-column reagent: 10 μM DiA in H2O; reagent flow rate: 0.62 mL/min; excitation/emission wavelengths: 450/570 nm.
