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LC-ESI/MS/MS traces of a 10 μM standard mixture of LPAs. Column: Luna™ C-8 (50 × 2 mm, 3 μm) at 40 °C. Injection volume: 10 μL. Mobile phase: 9:1 MeOH:aqueous HCOOH (pH 2.5) at a flow rate of 0.4 mL/min. Parent and daughter ions were detected in the negative ion mode, sprayer voltage; 3.0 kV, capillary temperature at 300 °C.
