Table 3.

Statistical values obtained for the individual LPA species in the LC-ESI/MS/MS method (n = 3).

LPA species	Retention time (min)	Linear range (μM)	R2	LOD (μM)
14:0	6.30	0–40	0.9991	0.0067
20:4	7.55	0–40	0.9990	0.0099
16:0	8.29	0–40	0.9998	0.0123
18:1	9.10	0–40	0.9993	0.0066
18:0	11.22	0–40	0.9991	0.0156

Native LPA concentrations were determined in blind human plasma samples from five different donors using both the MS and the optical methods. In addition, to further evaluate other potential matrix interferences, these plasma samples were also spiked with 0.5 μM of each LPA species. Data from the samples collected from donors B, C, D and E is presented in the electronic supplementary information (Tables S4–S7, ESI^†).