Figure 2. Gating hierarchy and Stats phosphorylation.

(A) Gating hierarchy in whole blood.. An average of about 11,000 events were collected for CD33⁺ monocytes, 4500 for CD20⁺ B cells, 30,000 for CD3⁺ T cells, 20,000 for CD3⁺CD4⁺ T cells, 7500 for CD3⁺CD8⁺ T cells, and 120,000 CD33⁺ granulocytes. (B) Histogram overlays of fluorescent intensities of antibody conjugated fluorochromes specific for indicated phosphorylated Stat proteins are shown before and after IFN-β administration. Phosphorylation levels for Stat proteins are shown in CD33⁺ monocytes and CD3⁺ T cells. Increased intensities are represented in yellow histograms.