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. 2014 Mar 1;141(5):1036–1046. doi: 10.1242/dev.106534

Fig. 3.

Fig. 3.

Erm restricts the developmental potential in immature INPs by repressing gene transcription. (A) Schematics of the UAS-erm transgenes used in this series of experiments. ERD, engrailed repressor domain; VP16, transactivation domain. (B) Overexpression of erm induces premature neuroblast differentiation by repressing gene transcription. Third instar larval brains of the indicated genotype were stained for Dpn, Ase and Phall, and the total number of type II neuroblasts (Dpn+Ase-) per brain lobe was quantified. (C) Restoring erm expression rescues the supernumerary neuroblast phenotype in erm-null brains by repressing gene transcription. Third instar larval brains of the indicated genotypes were treated and analyzed as in B. (D) Overexpression of VP16-ermzf exerts a dominant-negative effect and further exacerbates the supernumerary neuroblast phenotype in erm hypomorphic brains. Third instar larval brains of the indicated genotypes were treated and analyzed as in B.