Table 2. 2’-F-modified RNA aptamers binding to Hyper-IL-6.

Aptamer	Sequence	Frequency	Kd value [nM]
FAIR-1	AGGAUGGCAA UCAGUGAACG GAAGGUGUAG GGUUAGAGGU GUGGUGGGUA	1	179.1 ± 37.4
FAIR-4	CUCGAGAGGC GUUGAUCGGA AGGCGGUAGG UGUUAUGGGU GGGAGGGAGC	2	120.0 ± 36.4
FAIR-6	GUAAGUAGUG UAGGCUGUGG GAGUUAUAGGGGUGGAUGUGGAGUGGGGUG	4	40.9 ± 12.7
FAIR-7	UGUCAGUAGC ACCAGUGGCG GACAGUAGGG CAGGGUGGAG UGGGUGUCCC	1	79.7 ± 33.2
FAIR-8	CGAGGCGCCG UGGAACAAGU AGGCUGUUAG GGAAGGGUGG AGCGGGUAGC	2	196.7 ± 27.9
FAIR-14	GAAGGCAGUG UGUAGUGCGG AGGUAGUUGA GUGGUGGGAG GGUGGAGGUA	1	118.9 ± 63.6
FAIR-15	GGGAUGACGG UAGGGUAGGG GGGUGGGGGU CAUCAAUUGG GAGGUAG	1	162.9 ± 69.0

Sequences are printed in 5′–3′ direction omitting flanking primer-binding sites. Bold letters highlight the conserved G-rich motif 5′-GGGGHGGCWG UGGWGWGGG-3′, which had already been identified in unmodified sIL-6R-binding RNA aptamers.²⁰