Figure 1. Creation of IgG1 Fc expressing tumor cells. (A) A construct expressing IgG1 Fc‐transferrin transmembrane region was subcloned into a retroviral vector expressing IRES‐GFP. EG7 cells were transduced with retro virus expressing either GFP containing empty vector (EG7-EV) or the IgG1 Fc construct EG7-Fc). (B) Lower panels show staining for GFP (X axis) and IgG1 (Y axis) in sorted cells that express just the empty vector (left panel) or the mTR-Fc construct (Right panel).