Figure 3. Dendritic cells exposed to EG7-Fc bearing tumors prime functionally superior CD8 T cells. GM‐CSF derived BMDCS were co‐incubated with either EG7-EV cells or EG7-Fc cells for a period of 12‐16 h. DCs were then sorted on a flow cytometer and incubated at 1:10 ratio with OT‐I T cells. After 3 d of culturing, CD8 T cells were assessed for intracellular IFN-gamma, TNF-α and Granzyme B (A). CD8 T cells were also incubated with target cells (EG7) at the indicated effector:target ratio for a period of 12 h to measure cytotoxicity (B). The data are representative of two independent experiments.