Figure 4. Enhanced CD8 T cell activation can be inhibited by blocking Fc receptors and enhanced by the addition of TLR3 agonist. (A) GM‐CSF BMDCs were pre‐incubated with a control antibody or a blocking antibody to Fc receptors (clone 2.4G2) before being cultured with either EG7-EV or EG7‐Fc cells. After overnight culture, DCs were sorted and plated at different concentrations before addition of OT‐I T cells. T cell proliferation was measured after 72 h as described previously. B‐C, Experiments performed as previously with the addition of 10 µM poly I:C to tumor‐BMDC cultures 8 h prior to incubation with OT‐I cells (B) and OT‐II cells (C). The data are representative of three independent experiments.