Figure 6. EG7-Fc tumor cells fail to grow in vivo and induce higher CD8 T cell responses. (A) Groups of 15 mice were implanted subcutaneously with 5 × 10⁵ tumor cells in the flanks. Five mice from each group were sacrificed on days 7, 14 and 21 and tumors were excised and weighed to measure growth. Mice that received empty vector expressing tumor cells grew large tumors by day 30, however mice that received mTR‐Fc cells failed to grow detectable tumors at day 30. Both groups had palpable and measurable tumors at days 7 and 14. (B) Draining lymph nodes (inguinal) were harvested and pooled from 5 mice for each group. CD8+ T cells were purified using negative selection and allowed to proliferate on BMDCs that had been cocultured with tumor cells for 12 h prior and purified by FACS. After 48 h of culture, CD8 T cell proliferation was measured by ³H-thymidine incorporation. The data are representative of three independent experiments and p values were determined by two-tailed unpaired t-test.