Figure 6. Cell binding of SH313-F9 variants. CD30+ Karpas299 cells were stained with a dilution series of IgGs which were converted from affinity matured scFv (A), scFabΔC (B), or FabΔC (C) candidates. The mean fluorescence intensities are summarized in the diagrams shown in the middle column. In addition, overlay histogram plots (right column) of CD30+ Karpas299 cells stained with 0.6 nM (C) or 1.3 nM (A and B) of representative affinity matured IgG candidates (red line) are shown in comparison to the parental SH313-F9 IgG (blue line). Cells only stained with secondary detection antibody conjugate are shown as negative control (black line).